Common Interview Questions and Answers on HPLC

What is the Full form of HPLC?

HPLC stands for High-performance Liquid Chromatography or High-Pressure Liquid Chromatography.

What is the HPLC Principle?

It is a chromatographic technic used to separate the components from a mixture of a solution by using a solid stationary phase and a liquid mobile phase.

How many types of chromatography?

There are 12 types of chromatography.

What are the types of Column Chromatography?

There are 5 types of chromatography methods where the column is used. 1. Liquid Chromatography 2. Gas Chromatography 3. Ion Exchange Chromatography 4. Size Exclusion Chromatography 5. Chiral Chromatography

What is the Column Chromatography Principle?

The column chromatography principle is, Column chromatography is fundamentally based on the adsorption of solutes from a solution by means of a stationary phase, which then separates each component of the mixture.

What is Reverse phase chromatography?

Mobile phase is more polar than the stationary phase is called reserve phase chromatography.

What is Normal phase chromatography?

Stationary phase is more polar than the mobile phase called normal phase chromatography.

What is Chiral Chromatography?

It is a chromatography to determine the content of chiral isomers whether it is in normal or reverse phase.

What is a Column?

A column is a still tube that contains a stationary phase.

What is the Stationary Phase?

The packing material for the column, which is the immobile phase in the chromatographic process, is known as the stationary phase.

What is the Mobile Phase?

The liquid that transports the solute through the column is known as the mobile phase.

What are the components of HPLC?

There are 6 types of components, 1. Reservoir 2. Pump 3. Sample Components 4. Column Components 5. Detector 6. Recorder.

What are the types of Detectors used in Chromatography?

There are 6 detectors are used in HPLC based on their usage, 1. UV detector 2. PDA Detector 3. Fluorescence detector 4. Conductivity detector 5. Refractive Index Detector 6. Light Scattering Detector.

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Resource Person: Mazhar Khan

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